Second professional interactive workshop for dental assistants were conducted on March 3, 2018, at the Ambitio Exercise Center for the participants who work in a team in dental office and who want to learn or renew their knowledge. Workshop topics were selected by dental assistants, who find such workshops an opportunity for learning, improving knowledge and exchanging experiences from the best colleagues. Workshop lecturers are people with great experience in their area of expertise. The topic on venepuncture and PRF preparatory clinical protocol was presented theoretically and practically by Maja Barošević, bacc. med. techn., from University Hospital Center Zagreb, “Emergency and Demonstration of Life Support Protocols in the Dental Office” presented by Goran Stokić, bacc. med. techn. spec., from the Institute of Emergency Medicine of the City of Zagreb, and “Dental Assisting in Orthodontics from First Examination to Orthodontic Device Design” was presented theoretically and practically by Marija Magdalenić-Meštrović, Ph.D., MD, spec. orthodontics, Head of Orthodontics Department at the Zagreb Dental Clinic. Dental assistants were satisfied with all knowledge provided and they expressed an interest for new workshops. The atmosphere from the workshop you can see in photo gallery.