Promotion of three generations of dental assistants were held at the Esplanade Hotel on September 24, 2021. The 13th and 14th generations of participants in the upskilling program for dental assistants and the 5th generation of the reskilling program for dental assistants were promoted by certificates and the first school textbook on dental assistance. The director of Ambitio College, Alen Stranjik, addressed participants in a short speech, congratulating them and saying that although they have completed their education as a dental assistant, they should continue their education and work in a profession that is constantly evolving and advancing. Promotion was held within the framework of the congress organized by Croatian Chamber of Dental Medicine “New Trends in Dental Medicine”. Professor Pavel Kobler, PhD on behalf of Croatian Chamber of Dental Medicine also congratulated them and invited everyone to join the class of dental assistants at the Chamber and to attend congresses throughout the year where they can hear interesting lectures for dental assistants.